1.
A quantitative liposome microarray to systematically characterize protein-lipid interactions.
Saliba AE, Vonkova I, Ceschia S, Findlay GM, Maeda K, Tischer C,
Deghou S,
van Noort V,
Bork P, Pawson T, Ellenberg J, Gavin AC
Nat Methods.
2014 Jan 24; 11(1): 47-50. Epub 2013 Nov 24; PubMed:
24270602.Abstract + PDF
Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.