A quantitative liposome microarray to systematically characterize protein-lipid interactions.
Saliba AE, Vonkova I, Ceschia S, Findlay GM, Maeda K, Tischer C, Deghou S
, van Noort V
, Bork P
, Pawson T, Ellenberg J, Gavin AC
2014 Jan 24; 11(1): 47-50. Epub 2013 Nov 24; PubMed: 24270602.
Abstract + PDF
Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.